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Methyl Green Counterstain Protocol

Introduction Methyl green is used commonly with bright-field microscopes to stain cell nuclei by dyeing the chromatin of cells  with a green color. It is chemically related to cresyl violet. Materials 0.1M Sodium Acetate Buffer, pH4.2 Sodium acetate, trihydrate (MW 136.1) —— 1.36 gDistilled water ———————————– 100 mlMix to dissolve and adjust pH to 4.2  Full Article…

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Gallyas Silver Stain

The Gallyas Silver stain is used in neuropathology for the detection of neurofibrillary tangles, associated with neurodegenerative disorders like Alzheimer’s disease.

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Weil’s myelin stain

Weil’s stain is a simple technique that is used to stain myelin in the nervous system, involving the reduction of chrome salt to chromium dioxide by myelin.

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