A SDS antigen retrieval solution can be used on formalin or paraformaldehyde-fixed tissues that have been sectioned on a cryostat; typically such tissues are cryoprotected using 30% sucrose . A brief exposure to SDS has been reported to greatly improve staining for some proteins.
1% Sodium Dodecyl Sulfate (SDS) in Phosphate Buffered Saline
- Mix 1.0 g of SDS in 100 ml of 0.01 M phosphate buffered saline (PBS).
- Store at room temperature for up to 3 months; for extended storage, store at 4oC.
- Rinse sections three times for 5 minutes each in PBS.
- Cover sections with 1% SDS solutions and incubate for 5 minutes at room temperature with gentle rocking.
- Wash sections in three changes of PBS , for 5 minutes each change, to eliminate remaining SDS solution. (It is critical to remove residual SDS as this will denature antibodies, preventing effective binding.)
- Proceed with tissue blocking for immunohistochemistry.
- Brown D, Lydon J, McLaughlin M, Stuart-Tilley A, Tyszkowski R, Alper S (1996) Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS).Histochem Cell Biol. 105(4):261-7. PubMed Abstract