This YouTube video provides an overview of methods for preparing tissue for paraffin sectioning.
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Polylysine-coated tissue culture surfaces
Polylysine solutions are typically used to coat glassware and plasticware for the attachment and growth of cells.
Tris is used to make a basic buffering solution.
Carbonate buffers are a combination of a weak acid and its conjugate salt, relying upon series of reverse reactions to buffer changes in pH.
Tris-Buffered Saline (TBS)
Tris-buffered saline (TBS) is isotonic, notoxic buffer used in some biochemical techniques that is maintain the pH within a relatively narrow range.
Acid precipitation solution
Acid precipitation solutions can be used to precipitate nucleic acids.
Making stock acid and base solutions (molarities and specific gravities of concentrated acids and bases)
A useful reference table for making common acid and base stock solutions.
Coomassie Brilliant Dyes are a family of dyes commonly used to stain proteins in sodium dodecyl sulfate and blue native polyacrylamide gel electrophoresis gels.
Paraffin Processing of Tissue
Learn about the technique of paraffin embedding, a classic method used in histology.
Haematoxylin Eosin (H&E) staining
H&E stains are the most common staining technique used for histologic studies.