Find out about mordants, substance used to set dyes on tissue sections.
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PMSF
PMSF is commonly used in protein solublization in order to deactivate proteases from digesting proteins of interest after cell lysis.
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Ampicillin
Ampicillin is a beta-lactam antibiotic used as a selective agent in molecular biology to select for the uptake of genes by bacteria.
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Acrylamide
Acrylamide solution is used for the creation of polyacrylamide gels in gel electrophoresis techniques, such as western blotting.
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Two-dimensional gel electrophoresis
Learn about two-dimensional gel electrophoresis, a form of gel electrophoresis commonly used to analyze multiple characteristics of proteins.
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One-Dimensional SDS Gel Electrophoresis of Proteins (western blotting)
Learn about western blotting, an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.
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Good’s buffers
Good’s buffers are twelve buffering agents selected and described by Norman Good and colleagues in 1966. Good selected the buffers based on a number of criteria which make them candidates for use in biochemistry and biological research. Many remain staples in modern biology laboratories. Selection criteria Good sought to identify buffering compounds which met several Full Article…
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An inexpensive mounting medium for microscopy
Here’s an inexpensive semi-permanent mounting media for immunofluorescence.
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Weil’s myelin stain
Weil’s stain is a simple technique that is used to stain myelin in the nervous system, involving the reduction of chrome salt to chromium dioxide by myelin.
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Lysogeny broth
Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. It is more commonly known as Luria broth or Luria-Bertani broth.
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