Although chlorine bleach is commonly employed in many laboratories as a neutralization procedure, it is not effective in removing the mutagenic properties of DAB. A potassium permanganate-sulfuric acid procedure is, however, an effective way of neutralizing this toxic compound.
- Take up bulk quantities of diaminobenzidine tetrahydrochloride dehydrate in water and bulk quantities of the free base in 0.1 M hydrochloric acid so that the concentration of DAB does not exceed 0.9 mg/ml. Dilute solutions with the same buffer, if necessary, so that the concentration does not exceed 0.9 mg/ml.
- For each 10 ml of solution, add 5 ml of 0.2 M potassium permanganate solution (31.6 g KMnO4 per liter of solution with water) and 5 ml of 2 M sulfuric acid solution (112 ml concentrated H2S04 per liter of solution with water).
- Allow the mixture to stand for at least 10 hours.
- Test to ensure pH of solution is between 6-9. Dispose solution down the drain with copious amounts of water.