Introduction Methyl green is used commonly with bright-field microscopes to stain cell nuclei by dyeing the chromatin of cells with a green color. It is chemically related to cresyl violet. Materials 0.1M Sodium Acetate Buffer, pH4.2 Sodium acetate, trihydrate (MW 136.1) —— 1.36 gDistilled water ———————————– 100 mlMix to dissolve and adjust pH to 4.2 Full Article…
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DAB-Peroxidase Substrate Solution (Brown)
Learn how to make DAB for immunohistochemical staining.
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Avidin-Biotin Blocking Solution
Avidin-Biotin blocking solution is used to reduce non-specific background that is commonly associated with biotinylated antibodies.
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SDS Antigen Retrieval Solution
A SDS antigen retrieval solution can dramatically improve staining in paraformaldehyde-fixed, cryostat-sectioned tissue.
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Tris Buffer Antigen Retrieval Protocol
A Tris Buffer antigen retrieval solution is a basic pH solution that is used to improve staining in paraffin embedded, formalin or paraformaldehyde-fixed tissues
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Tris Buffered Saline (TBS) Antigen Retrieval Protocol
A Tris Buffered Saline (TBS) antigen retrieval solution is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues.
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Tris-EDTA Antigen Retrieval Protocol
A Tris-EDTA buffer antigen retrieval solution is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues.
EDTA Buffer Antigen Retrieval Protocol
EDTA buffer is an antigen-retrieval solution used to improve staining for low abundance epitopes.
Citrate-EDTA Antigen Retrieval Protocol
Citrate EDTA buffer antigen retrieval solution is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues.
Citrate Buffer Antigen Retrieval Protocol
Citrate buffer antigen retrieval is a solution used at elevated temperatures to improve immunohistochemical staining in fixed tissues.