Carbonate buffer

Carbonate buffers are a combination of a weak acid and its conjugate salt, relying upon series of reverse reactions to buffer changes in pH.

To make 1 liter of carbonate buffer, add the following

  • 1.6 g Na2CO3 (15 mM final)
  • 2.9 g NaHCO3 (35 mM final)
  • 0.2 g NaN3 (3.1 mM final). Use caution in handling sodium azide as it is extremely  poisonous; follow appropriate precautions for handling,
    storage, and disposal.
  • H2O to 1 liter

Mix well, and then adjust to pH 9.5.

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